epithelial cells primary lung atcc pcs 300 010  (ATCC)

Journal: Translational Oncology

Article Title: Clinical significance and molecular mechanism of CDX2-CBX3 regulatory axis in lung adenocarcinoma progression

doi: 10.1016/j.tranon.2025.102590

Figure Lengend Snippet: CBX3 and CDX2 promote LUAD cell proliferation and colony formation (A) Box plot illustrating differential expression of CDX2 between LUAD tumors ( n = 483) and normal lung tissues ( n = 59) from the TCGA dataset. (B) Kaplan-Meier survival analysis demonstrating the association between CDX2 expression levels and survival probabilities in patients with LUAD. (C) qPCR analysis of CBX3 and (D) CDX2 mRNA expression in normal lung epithelial cells (HSAEC1-KT) and four LUAD cell lines (H2106, H2023, H1623, H2347), ** P < 0.01 versus HSAEC1-KT ( n = 6). (E) CCK-8 proliferation assay in H2023 cells after transfection with si-CBX3 or si-CDX2 ( n = 6). (F) CCK-8 proliferation assay in H2347 cells following transfection with OE-CBX3 or OE-CDX2 ( n = 6). (G) Representative images of colony formation assays. (H) Quantification of colony formation in H2023 cells and (I) H2347 cells ( n = 6), ## P < 0.01 versus si-NC or OE-NC. (J) Representative images of western blotting of CBX3 and CDX2 expression in normal samples or samples from patients with primary lung cancer. (K-L) Relative expression level of CBX3 and CDX2 expression in normal samples or samples from patients with primary lung cancer ( n = 8), ⁎⁎ P < 0.01 versus Normal.

Article Snippet: Normal lung epithelial cells HSAEC1-KT and human LUAD cell lines H2106, H2023, H1623, and H2347 (all from ATCC) were cultured in complete 1640 medium (Gibco) supplemented with 10 % fetal bovine serum (FBS) and 1 % penicillin-streptomycin (Beyotime) at 37 °C and 5 % CO 2 .

Techniques: Quantitative Proteomics, Expressing, CCK-8 Assay, Proliferation Assay, Transfection, Western Blot